Journal: Antibiotics

Article Title: New Antimicrobial Bioactivity against Multidrug-Resistant Gram-Positive Bacteria of Kinase Inhibitor IMD0354

doi: 10.3390/antibiotics9100665

Figure Lengend Snippet: IMD0354 does not show hemolytic activity. Human red blood cells were incubated with serially diluted IMD0354 (0.0156–16μg/mL) and normalized to hemolysis of 1% Triton-X 100. Each sample was tested in triplicate ( n = 3, ± S.D).

Article Snippet: 10% human erythrocytes were purchased from Rockland Immunochemicals (Limerick, PA, USA).

Techniques: Activity Assay, Incubation

Journal: bioRxiv

Article Title: Separability of antibacterial and membranolytic activity in the human host defense peptide LL-37

doi: 10.1101/2025.05.22.655608

Figure Lengend Snippet: A. Size exclusion chromatography (SEC) of LL-37 surface mutants as in . B. SEC of overlapping mutants from . C. Biolayer interferometry (BLI) showing the shifts associated with incubating the indicated analyte with a probe consisting of biotinylated LL-37. D. Extension of hemolysis assays to the sample types shown. Sheep and cow blood are defibrinated. Human blood is purchased whole blood from each of two donors. Sheep and human RBCs indicate isolated RBCs. E. Luminescence toxicity assay with each of the indicated cell types.

Article Snippet: Types of blood used in these studies included defibrinated sheep and cow blood (Hardy Diagnostics), purchased human whole blood from two separate donors (ZenBio), sheep RBCs (Innovative Research), and human RBCs (Rockland Immunochemicals).

Techniques: Size-exclusion Chromatography, Isolation